I changed a bit and it worked like this, dumped into one single array, provided all the images are of same dimensions.

png = []
for image_path in glob.glob("./train/*.png"):
    png.append(misc.imread(image_path))    

im = np.asarray(png)

print 'Importing done...', im.shape

According to the doc, scipy.misc.imread is deprecated starting SciPy 1.0.0, and will be removed in 1.2.0. Consider using imageio.v3.imread instead.

Example:

import imageio.v3 as iio

im = iio.imread('my_image.png')
print(im.shape)

You can also use imageio to load from fancy sources:

im = iio.imread('http://upload.wikimedia.org/wikipedia/commons/d/de/Wikipedia_Logo_1.0.png')

Edit:

To load all of the *.png files in a specific folder, you could use the glob package:

import imageio.v3 as iio
import glob

for im_path in glob.glob("path/to/folder/*.png"):
     im = iio.imread(im_path)
     print(im.shape)
     # do whatever with the image here

This can also be done with the Image class of the PIL library:

from PIL import Image
import numpy as np

im_frame = Image.open(path_to_file + 'file.png')
np_frame = np.array(im_frame.getdata())

Note: The .getdata() might not be needed – np.array(im_frame) should also work

If you are loading images, you are likely going to be working with one or both of matplotlib and opencv to manipulate and view the images.

For this reason, I tend to use their image readers and append those to lists, from which I make a NumPy array.

import os
import matplotlib.pyplot as plt
import cv2
import numpy as np

# Get the file paths
im_files = os.listdir('path/to/files/')

# imagine we only want to load PNG files (or JPEG or whatever...)
EXTENSION = '.png'

# Load using matplotlib
images_plt = [plt.imread(f) for f in im_files if f.endswith(EXTENSION)]
# convert your lists into a numpy array of size (N, H, W, C)
images = np.array(images_plt)

# Load using opencv
images_cv = [cv2.imread(f) for f in im_files if f.endswith(EXTENSION)]
# convert your lists into a numpy array of size (N, C, H, W)
images = np.array(images_cv)

The only difference to be aware of is the following:

• opencv loads channels first
• matplotlib loads channels last.

So a single image that is 256*256 in size would produce matrices of size (3, 256, 256) with opencv and (256, 256, 3) using matplotlib.

Using a (very) commonly used package is prefered:

import matplotlib.pyplot as plt
im = plt.imread('image.png')

I like the build-in pathlib libary because of quick options like directory= Path.cwd()
Together with opencv it’s quite easy to read pngs to numpy arrays.
In this example you can even check the prefix of the image.

from pathlib import Path
import cv2
prefix = "p00"
suffix = ".png"
directory= Path.cwd()
file_names= [subp.name for subp in directory.rglob('*') if  (prefix in subp.name) & (suffix == subp.suffix)]
file_names.sort()
print(file_names)

all_frames= []
for file_name in file_names:
    file_path = str(directory / file_name)
    frame=cv2.imread(file_path)
    all_frames.append(frame)
print(type(all_frames[0]))
print(all_frames[0] [1][1])

Output:

['p000.png', 'p001.png', 'p002.png', 'p003.png', 'p004.png', 'p005.png', 'p006.png', 'p007.png', 'p008.png', 'p009.png']
<class 'numpy.ndarray'>
[255 255 255]

To read in one image:

import PIL.Image
im = PIL.Image.open('path/to/your/image')
im = np.array(im)

Iterate to read in multiple images.

This answer is similar to this but simpler (no need for .getdata()).

If you prefer the standard library:

#IMPORTANT: This Code only works with Python>=3.6
Directory="."#Your directory
import os
import tkinter
import numpy
tk=tkinter.Tk()
tk.overrideredirect(1)
tk.geometry("0x0")
Items=[]
for i in os.listdir(Directory):
    fn=Directory+os.sep+i
    imgArray=[]
    image=tkinter.PhotoImage(file=fn)
    for w in range(image.width()):
        entry=[]
        for h in range(image.height()):
            entry.append(image.get(w,h))
        imgArray.append(entry)
    imgArray=numpy.array(imgArray)
    Items.append(imgArray)
tk.destroy()